Higher nisin yield is reached with glutathione and pyruvate compared with heme in Lactococcus lactis N8
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چکیده
منابع مشابه
Enhancement of Nisin Production by Lactococcus lactis subsp. lactis.
Lactococcus lactis subsp lactis BSA (L. lactis BSA) was isolated from a commercial fermented product (BSA Food Ingredients, Montreal, Canada) containing mixed bacteria that are used as starter for food fermentation. In order to increase the bacteriocin production by L. lactis BSA, different fermentation conditions were conducted. They included different volumetric combinations of two culture me...
متن کاملEvidence for a role of NisT in transport of the lantibiotic nisin produced by Lactococcus lactis N8.
The biosynthesis, immunity and regulation of nisin, a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis, is encoded by two gene clusters, nisA/ZBTCIPRK and nisFEG. The mutant strain LAC46 with a deletion in the translocator gene nisT could not secrete nisin but nisin activity was detected from cell lysates. The nisT mutation was complemented by a NisT-expression plasmi...
متن کاملDistribution and evolution of nisin-sucrose elements in Lactococcus lactis.
The distribution, architecture, and conjugal capacity of nisin-sucrose elements in wild-type Lactococcus lactis strains were studied. Element architecture was analyzed with the aid of hybridizations to different probes derived from the nisin-sucrose transposon Tn5276 of L. lactis NIZO R5, including its left and right ends, the nisA gene, and IS1068 (previously designated iso-IS904), located bet...
متن کاملRegulation of nisin biosynthesis and immunity in Lactococcus lactis 6F3.
The biosynthetic genes of the nisin-producing strain Lactococcus lactis 6F3 are organized in an operon-like structure starting with the structural gene nisA followed by the genes nisB, nisT, and nisC, which are probably involved in chemical modification and secretion of the prepeptide (G. Engelke, Z. Gutowski-Eckel, M. Hammelmann, and K.-D. Entian, Appl. Environ. Microbiol. 58:3730-3743, 1992)....
متن کاملEnhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44
Traditionally, nisin was produced industrially by using Lactococcus lactis in the neutral fermentation process. However, nisin showed higher activity in the acidic environment. How to balance the pH value for bacterial normal growth and nisin activity might be the key problem. In this study, 17 acid-tolerant genes and 6 lactic acid synthetic genes were introduced in L. lactis F44, respectively....
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2020
ISSN: 1517-8382,1678-4405
DOI: 10.1007/s42770-019-00216-w